The Lobed-Leaf Phenotype in Brassica juncea Is Associated with the BjLMI1 Locus as Evidenced Using GradedPool-Seq

The shape of the leaf is primary phenotype which determines commercial value mustard (Brassica juncea). However, there arefew reports on lobed-leaf gene B. juncea, and molecular regulatory mechanisms underlying margin formation are unknown. In this study, an F2 population derived from ‘MN001’ ‘MU056’ was constructed. Genetic analysis revealed that trait controlled by a major gene, lobed leavesare dominant compared to round leaves. GradedPool-Seq combined with re-sequencing results parents identified interval chromosome 10 juncea’s genome A. BjLMI1 (BjuA040054) confirmed be candidate ontology (GO) analysis, it homologous LMI1 encodes HD-Zip protein ATHB-51. A base substitution observed in conserved domain, 63 bp fragment deletion found exon region between two CDs region. expression significantly higher parent than round-leaf parent. These findings provide insights into mechanism will valuable development ideal juncea.